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Office of Research Services
A Division of the Office of the Vice President for Research
 
 

Manager
Jeff Wagner
jrwagner@uga.edu
Phone: 706-542-6409
Toll-free: 866-410-6935
Fax: 706-542-6414

Sequencing and
Synthesis Facility

University of Georgia
Riverbend Research Lab
Room 161
110 Riverbend Rd
Athens, GA 30602

 
 
 
 

Services and Pricing

Quick Links:

DNA Synthesis

Stock Primers

We have the following primers on hand that we will supply for sequencing if so desired. Please verify that the sequence of the primer listed below is the one that you need. Also, make sure that the primer is at least 50 bases away from the region that you are interested in sequencing.

Primer Sequences
Primer Name Length Sequence
M13-20 FORWARD 18 TGT, AAA, ACG, ACG, GCC, AGT
M13-48 REVERSE 24 AGC, GGA, TAA, CAA, TTT, CAC, ACA, GGA
T7 PROMOTER 20 TAA, TAC, GAC, TCA, CTA, TAG, GG
T7 TERMINATOR 21 TTA, TGC, TAG, TTA, TTG, CTC, AGC
T3 17 AAC, CCT, CAC, TAA, AGG, GA
SK 17 TCT, AGA, ACT, AGT, GGA, TC
KS 17 CGA, GGT, CGA, CGG, TAT, CG
SP6 18 ATT, TAG, GTG, ACA, CTA, TAG

Oligo Technical Information

MOLECULAR WEIGHT OF AN OLIGONUCLEOTIDE:
MW = ((A x 312.2) + (G x 328.2) + (C x 288.2) + (T x 303.2) -61.0)

CONVERSION FACTORS:
1 OD260 unit = 33 micrograms of an oligonucleotide

STORAGE CONDITIONS:
Storage Conditions >> Stability
Lyophilized at -20C >> 6 months to several years
Lyophilized at 25C >> 2 months to 1 year
Dissolved at -20C >> 1 month to 6 months
Dissolved at 25C >> 1 week to 3 months

RESUSPENSION BUFFERS:
Sterile Water
TE Buffer (10uM Tris-HCL, 1mM EDTA)

IS PURIFICATION NECESSARY?
PCR reactions >> No, but if problems exist try purification.
DNA Sequencing >> No, but if problems exist try purification.
Mutagenesis >> Yes
Gene Construction >> Yes
Oligonucleotides greater than 30 bases >> Yes

DNA Sequencing

Sequencing Costs

The Sequencing and Synthesis Facility is offering several options for our DNA Sequencing customers.

  1. Our standard full service option at $17.00 per template/primer combination.
  2. $2.50 per sample for ready to run samples.
  3. $65.00 for a 96-well plate of ready to run samples.
  4. $10.00 per reaction (one well/one primer) for samples submitted in a 96-well plate.

Please contact Jeff for any questions about pricing and to discuss pricing for purification of plasmid and PCR products.


Peptide Synthesis

Synthesis Costs

Linear:

$9.00 per Amino Acid
Minimum charge of $90.00

Call 542-6409 for pricing of modifications.


Request Forms