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DNA Synthesis
Stock Primers
We have the following primers on hand that we will supply for sequencing if so desired. Please verify that the sequence of the primer listed below is the one that you need. Also, make sure that the primer is at least 50 bases away from the region that you are interested in sequencing.
Primer Sequences
| Primer Name | Length | Sequence |
| M13-20 FORWARD | 18 | TGT, AAA, ACG, ACG, GCC, AGT |
| M13-48 REVERSE | 24 | AGC, GGA, TAA, CAA, TTT, CAC, ACA, GGA |
| T7 PROMOTER | 20 | TAA, TAC, GAC, TCA, CTA, TAG, GG |
| T7 TERMINATOR | 21 | TTA, TGC, TAG, TTA, TTG, CTC, AGC |
| T3 | 17 | AAC, CCT, CAC, TAA, AGG, GA |
| SK | 17 | TCT, AGA, ACT, AGT, GGA, TC |
| KS | 17 | CGA, GGT, CGA, CGG, TAT, CG |
| SP6 | 18 | ATT, TAG, GTG, ACA, CTA, TAG |
Oligo Technical Information
MOLECULAR WEIGHT OF AN OLIGONUCLEOTIDE:
MW = ((A x 312.2) + (G x 328.2) + (C x 288.2) + (T x 303.2) -61.0)
CONVERSION FACTORS:
1 OD260 unit = 33 micrograms of an oligonucleotide
STORAGE CONDITIONS:
Storage Conditions >> Stability
Lyophilized at -20C >> 6 months to several years
Lyophilized at 25C >> 2 months to 1 year
Dissolved at -20C >> 1 month to 6 months
Dissolved at 25C >> 1 week to 3 months
RESUSPENSION BUFFERS:
Sterile Water
TE Buffer (10uM Tris-HCL, 1mM EDTA)
IS PURIFICATION NECESSARY?
PCR reactions >> No, but if problems exist try purification.
DNA Sequencing >> No, but if problems exist try purification.
Mutagenesis >> Yes
Gene Construction >> Yes
Oligonucleotides greater than 30 bases >> Yes
DNA Sequencing
Sequencing Costs
The Sequencing and Synthesis Facility is offering several options for our DNA Sequencing customers.
- Our standard full service option at $17.00 per template/primer combination.
- $2.50 per sample for ready to run samples.
- $65.00 for a 96-well plate of ready to run samples.
- $10.00 per reaction (one well/one primer) for samples submitted in a 96-well plate.
Please contact Jeff for any questions about pricing and to discuss pricing for purification of plasmid and PCR products.
Peptide Synthesis
Synthesis Costs
Linear:
$9.00 per Amino Acid
Minimum charge of $90.00
Call 542-6409 for pricing of modifications.
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